Microdissection

The microdissector PALM MicroBeam (Zeiss) can microdissect tissue sections or cultured live cells and catapult cut samples directly in the collector tube with the laser.

After microdissection, analyses of DNA, RNA, proteins or live cells can be performed.

System features

Light Sources :

HBO lamp

Halogen lamp

Laser for microdissection

Microscope: Zeiss Axio Observer Z1 inverted, entirely motorised + DIC

Cameras :

AxioCam MRm monochrome sensitive to deam lignt intensities

AxioCam CC1 color

APOTOME® structured light system

Objectives :

- Plan-Neofluar 2.5x/0.07

- Fluar 5x/0.25

- Plan-Neofluar 20x/0.4 Korr

- Plan-Neofluar 40x/0.6 Korr

Motorised stage in x, y, z

Filters :

- Zeiss set 49 DAPI : G 635, FT 395, BP 445/50

- Zeiss set 43 HE TRITC: BP550/25, FT 570, BP 605/70

- Zeiss set 38 HE FITC: BP 470/40, FT 495, BP 525/50

Softwares :

Palm Robo software 4.5 for microdissection

Axiovision 4.8 : modules of colocalisation, Inside4 (reconstitution 3D with surface rendering), MosaiX, Mark & find.

Accessories :

Robomover for sample collecting after catapulting

Collecters : 1x200, 8x200µl, livecell

Supports : slideholder triple, dishholder 50cc

microdissection of a renal glomerulus